Oct 15, 2019   7:19 p.m. Terézia
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Course syllabus N400M1_4D - Molecular Biology (FCFT - 2019/2020 - post-graduate studies)


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University: Slovak University of Technology in Bratislava
Faculty: Faculty of Chemical and Food Technology
Course unit title: Molecular Biology
Course unit code: N400M1_4D
Mode of completion and Number of ECTS credits: Exam-PhD (6 credits)
 
Name of lecturer: doc. RNDr. Helena Paulíková, CSc. (person responsible for course) - slovak, english
 
Learning outcomes of the course unit:
The lectures are aimed at students interested in furthering their understanding of how cells regulate and coordinate their core activities. The first part will be mainly background of gene regulation in eukaryotes and signalling cellular systems. The second part will focused on signalling defects and human disorders.
 
Prerequisites and co-requisites: none
 
Course contents:
The structure of DNA. Chromosome organization. Regulation of chromatin structure. Noncoding DNA. The structure and versatility of RNA.
DNA replication. DNA polymerases. The replication apparatus. Topoisomerases. The structure of telomeric DNA and telomerase. Telomeres, aging and cancer.
DNA recombination. Transposons. Mutation and repair of DNA.
Transcription in prokaryotes. Regulation of transcription: role of σ (sigma) factors, activators and represors. Two-component system in bacteria. Attenuation. Antitermination.
Translation. Genetic code. Recoding. Function of tmRNA and stringent factors/RelA.
Transcription in eukaryotes. The complexity of the transcriptional apparatus in higher organisms. A focus on Pol II and basal transcription factors. The activation and structure of transcription factors. Transcription factors and eukaryotic gene control. Heterochromatin and epigenetic control of gene expression.
RNA processing and post-transcriptional gene regulation. Splicing, including alternative splicing; capping; polyadenylation. RNA editing.
Translation. Formation of initiation complex in eukaryotes: control of initiation and phosphorylation of eIF2. RNA interference.
Translocation of proteins - specific signal sequences. Cotranslation translocation into endoplasmic reticulum of proteins. Vesicular transport and sorting of proteins. Traffic between nucleus and cytoplasm. Protein import pathways into mitochondria: TIM and TOM complexes.Degradation of proteins: ubiquitination and proteasome.
Receptors - plasma membrane receptors and intracellular receptors. Proteins of signalling pathways and second messengers. Organization of signalling complexes. Integration of signals. Extracellular matrix and immune system. Signalling in the endocrine system. Principles of cell-cycle regulation. Apoptosis - the necessity for cell death in multicellular organisms. Importance of cell death in human disease - cancer and neurodegenerative diseases.
 
Recommended or required reading:
Basic:
BECKERMAN, M. Molecular and Cellular Signaling. USA: Springer Science+Business Media, Inc, 2010. 592 p. ISBN 978-1-4419-1966-3.
JOHNSON, A. -- ALBERTS, B. Molecular Biology of the Cell . New York and London: Garland Science, 2007. 1539 p. ISBN 978-0-8153-4106-2.

 
Planned learning activities and teaching methods: lectures
 
Assesment methods and criteria: exam
 
Language of instruction: Slovak, English
 
Work placement(s): There is no compulsory work placement in the course unit.


Last modification made by Ing. Tomáš Molnár on 04/26/2019.

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