Oct 16, 2019   1:38 p.m. Vladimíra
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Course syllabus 43113_4B - Bioanalytical Methods I (FCFT - WS 2019/2020)


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University: Slovak University of Technology in Bratislava
Faculty: Faculty of Chemical and Food Technology
Course unit title: Bioanalytical Methods I
Course unit code: 43113_4B
Mode of completion and Number of ECTS credits: Exam (3 credits)
 
Name of lecturer: Dr.h.c. prof. Ing. Ján Šajbidor, DrSc. (person responsible for course) - slovak, english
 
Learning outcomes of the course unit:
Bioanalytical methods as a tool for the study of chemical composition of living objects (mostly microorganisms), but also the use of microbiological and biochemical methods for the determination of chemical substances in the environment.
 
Prerequisites and co-requisites: none
 
Course contents:
Homogenization of samples, preparation for analysis, Precipitation and desolvatation of macromolecules, Dialysis, lyophilization , Separation of components in centrifugal field, Basic physical principles, sedimentation and centrifugation, Gradient centrifugation, Flow centrifugation and centrifugation in zonal rotors, Electromigration methods, Basic physical principles, Paper electrophoresis, various types of gel electrophoresis, PAGE, Isotachophoresis, Isoelectric focusation, Radionuclide methods, Basic physical principles, the definition of radioactivity, Methods for the preparation of radiolabelled substances, Methods for detection of radiation, RIA methods and their application in analytical chemistry, Immunochemical analytical methods, The basic principles of the method, Preparation of polyclonal and monoclonal antibodies, EIA, ELISA and other immunoassay techniques, Affinity chromatography, Histochemical and cytochemical methods, The basic principles of the methods, Isolation and fixation of biological material, Ultrathin sections preparation, Histochemical methods for the analysis of proteins, saccharides, lipids and nucleic acids.
 
Recommended or required reading:
Basic:
RAUCH, P. -- FUKAL, L. -- KRÁLOVÁ, B. Bioanalytické metody. Praha: VŠCHT, 2001. 254 p. ISBN 80-708-0449-1.

 
Planned learning activities and teaching methods: The course contains 13 lectures, each taking 2 hours.
 
Assesment methods and criteria: The final evaluation of the student's knowledge is exam.
 
Language of instruction: Slovak, English
 
Work placement(s): There is no compulsory work placement in the course unit.


Last modification made by Ing. Tomáš Molnár on 01/29/2019.

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