Oct 17, 2019   3:32 a.m. Hedviga
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Course syllabus 43113_4B - Bioanalytical Methods I (FCFT - WS 2019/2020)

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University: Slovak University of Technology in Bratislava
Faculty: Faculty of Chemical and Food Technology
Course unit code: 43113_4B
Course unit title: Bioanalytical Methods I
Mode of completion and Number of ECTS credits: Exam (3 credits)
Course contents:
1.Basic methods for the isolation of substances from biological material (allowance 6/0)
a.Homogenization of samples, preparation for analysis
b.Precipitation and desolvatation of macromolecules
c.Dialysis, lyophilization

2.Separation of components in centrifugal field (allowance 4/0)
a.Basic physical principles, sedimentation and centrifugation
b.Gradient centrifugation
c.Flow centrifugation and centrifugation in zonal rotors

3.Electromigration methods (allowance 4/0)
a.Basic physical principles
b.Paper electrophoresis, various types of gel electrophoresis, PAGE
d.Isoelectric focusation

4.Radionuclide methods (allowance 4/0)
a.Basic physical principles, the definition of radioactivity
b.Methods for the preparation of radiolabelled substances
c.Methods for detection of radiation
d.RIA methods and their application in analytical chemistry

5.Immunochemical analytical methods (allowance 4/0)
a.The basic principles of the method
b.Preparation of polyclonal and monoclonal antibodies
c.EIA, ELISA and other immunoassay techniques
d.Affinity chromatography

6.Histochemical and cytochemical analysis methods (allowance 4/0)
a.The basic principles of the methods
b.Isolation and fixation of biological material
c.Uultrathin sections preparation
d.Histochemical methods for the analysis of proteins, saccharides, lipids and nucleic acids

Last modification made by Ing. Tomáš Molnár on 01/29/2019.

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