8. 12. 2019  13:34 Marína
Akademický informačný systém

Sylabus predmetu 43122_4B - Principles in Recombinant Technologies (FCFT - SS 2019/2020)

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University: Slovak University of Technology in Bratislava
Faculty: Faculty of Chemical and Food Technology
Course unit title: Principles in Recombinant Technologies
Course unit code: 43122_4B
Mode of completion and Number of ECTS credits: Exam (3 credits)
Name of lecturer: doc. Ing. Milan Čertík, PhD. (person responsible for course) - slovak, english
Learning outcomes of the course unit:
To gain the basic knowledge from recombinant technologies and methods used for clonning.
Prerequisites and co-requisites: (passed Fundamentals of General Microbiology and passed Fundamentals of Biochemistry) or (passed Microbiology I and passed Biochemistry I)
Course contents:
Characterization of gene manipulation and its relationship with other fields. DNA and RNA isolation from cells. Bacic enzyme type used in recombinant Technologies, construction of restriction maps. Properties and construction of vector systems, DNA introduction to cells and indentification of recombinant clones. Preparation and application of genomic and cDNA libraries. Techniques of selection and localization of genes in DNA molecules. Principles and anpplication of bacit techniques (gele, electrophoresis, radio- and nonradio-labelling DNA, hybridization, PCR, sequencing DNA). Genecically modified organisms.
Recommended or required reading:
BROWN, T A. Gene Cloning and DNA analysis : An introduction. 6th edition. Hoboken, NJ 07030-5774, USA: Wiley & Blackwell, 2010. 318 p. ISBN 978-1-4051-8173-0.

Planned learning activities and teaching methods: The subject is performed by lectures and seminars
Assesment methods and criteria: the fnal exam
Language of instruction: Slovak, English
Work placement(s): There is no compulsory work placement in the course unit.

Last modification made by Ing. Tomáš Molnár on 02/21/2019.

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