Sep 16, 2019   2:21 p.m. Ľudomila, Ľudmila
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Course syllabus N431Z0_4I - Fundamentals of Recombinant Technologies (FCFT - SS 2018/2019)


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University: Slovak University of Technology in Bratislava
Faculty: Faculty of Chemical and Food Technology
Course unit code: N431Z0_4I
Course unit title: Fundamentals of Recombinant Technologies
Mode of delivery, planned learning activities and teaching methods:
lecture2 hours weekly (on-site method)

 
Credits allocated: 2
 
Recommended semester/trimester: Food, Hygiene, Cosmetics - master (semi-compulsory), 2. semester
Nutrition and Food Quality Assessment - master (semi-compulsory), 2. semester
Level of study: 2.
Prerequisites for registration: none
 
Assesment methods:
Presence and successfully done test exams during semester
 
Learning outcomes of the course unit:
To gain the basic knowledge from recombinant technologies and methods used for clonning.
 
Course contents:
1.Introduction to recombinant technologies, structures and expression of nucleotide acids
2.Isolation and purification of DNA molecules
3.Basic characterization of enzymes used for DNA manipulation and separation
4.Plasmids as cloning vectors
5.Bacteriophages as cloning vectors
6.Introduction of DNA into eukaryotic cells
7.Labelling and hybridization of nucleic acids
8.Properties, preparation and application of DNA libraries
9.Obtaining specific gene
10.Localization of gene in DNA molecule
11.Basis of PCR
12.Sequencing DNA
13.Genetically modified organisms
 
Recommended or required reading:
Basic:
BROWN, T A. Gene Cloning and DNA analysis : An introduction. 6th edition. Hoboken, NJ 07030-5774, USA: Wiley & Blackwell, 2010. 318 p. ISBN 978-1-4051-8173-0.

 
Language of instruction: slovak or english
 
Notes:
 
Courses evaluation:
Assessed students in total: 3

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Name of lecturer(s): doc. Ing. Milan Čertík, PhD. (person responsible for course) - slovak, english
 
Last modification: 19. 2. 2018
Supervisor: doc. Ing. Milan Čertík, PhD. and programme supervisor


Last modification made by Ing. Tomáš Molnár on 02/19/2018.

Type of output: