18. 2. 2020  10:35 Jaromír
Akademický informační systém

Sylabus předmětu N426L0_4I - Laboratory of Biochemistry (FCFT - WS 2019/2020)

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University: Slovak University of Technology in Bratislava
Faculty: Faculty of Chemical and Food Technology
Course unit code: N426L0_4I
Course unit title: Laboratory of Biochemistry
Mode of delivery, planned learning activities and teaching methods:
laboratory/construction practice2 hours weekly (on-site method)

Credits allocated: 2
Recommended semester/trimester: Biotechnology - master (compulsory), 1. semester
Biotechnology - master (compulsory), 1. semester
Level of study: 2.
Prerequisites for registration: none
Assesment methods:
Final assessment will be made by evaluation of protocols and written tests.
Learning outcomes of the course unit:
The goal of this course is to teach students the methods for the isolation and analysis of proteins, DNA, and with methods of determination of enzymatic activity, and isolation of cell organelles.
Course contents:
Students will perform following experimental activities and measurements according to the instructions:
1. Isolation and electrophoretic separation of milk proteins.
2. Isolation and electrophoretic characterisation of plasmid DNA.
3. Determination of kinetic parameters of lactate dehydrogenase.
4. Purification of alfa-amylase and measurement of its activity by means of chromolytic substrate.
5. Isolation of chloroplasts and measurement of Hill reaction.
Students will summarize their results in protocols.
Recommended or required reading:
VAREČKA, Ľ. -- MIKO, M. Laboratórne cvičenia z biochémie II - skriptá. Bratislava: Vydavateľstvo STU, 1998.

Language of instruction: slovak or english
Courses evaluation:
Assessed students in total: 147

18,4 %27,2 %31,3 %17,7 %4,8 %0,6 %
Name of lecturer(s): Ing. Helena Galádová (examiner, instructor)
Ing. Zoltán Polozsányi (examiner, instructor)
doc. Ing. Martin Šimkovič, PhD. (person responsible for course)
Last modification: 29. 1. 2019
Supervisor: doc. Ing. Martin Šimkovič, PhD. and programme supervisor

Last modification made by Ing. Tomáš Molnár on 01/29/2019.

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