Dec 6, 2019   5:29 p.m. Mikuláš
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Course syllabus N418L10_4B - Laboratory of Instrumental Methods in Analysis (FCFT - WS 2019/2020)

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University: Slovak University of Technology in Bratislava
Faculty: Faculty of Chemical and Food Technology
Course unit code: N418L10_4B
Course unit title: Laboratory of Instrumental Methods in Analysis
Mode of delivery, planned learning activities and teaching methods:
laboratory/construction practice3 hours weekly (on-site method)

Credits allocated: 3
Recommended semester/trimester: Automation, Information Engineering and Management in Chemistry and Food Industry - bachelor (compulsory), 5. semester
Biotechnology - bachelor (compulsory), 5. semester
Food, Nutrition, Cosmetics - bachelor (compulsory), 5. semester
Chemical Engineering - bachelor (compulsory), 5. semester
Level of study: 1.
Prerequisites for registration: none
Assesment methods:
Practical execution of all laboratory exercises and test.
Learning outcomes of the course unit:
Provide advanced work using laboratory analytical techniques, basics of proper implementation of the analytical operations, good laboratory practice. Know the principles of creation of analytic signal measuring instruments and principles of optimization of various modes of measurement. Control instrumental analytical procedures for obtaining information and procedures evaluation of measurement results.
Course contents:
1.Basic principles work in the analytical laboratory . Working with measures of weight and
volume. Preparation of solutions.

2.Optical methods -  
● Molecular absorption spectral analysis in visible and ultraviolet radiation
- spectrophotometric determination of salicylic acid in Acylpyrine
- spectrophotometric determination of iron in mineral water.
- spectrophotometric determination of nitrates in the UV spectrum
- spectrophotometric determination of copper
● Atomic emission spectral analysis
- determination of sodium in water by flame photometry
● Nephelometry
- nefelometric determination of chlorides

3.Electroanalytical methods -
● Voltammetric titration with polarized electrodes
- iodometry with instrumental indication during titration
● Potentiometric titration
- determination of kys. Phosphate titration with sodium hydroxide to II. titration degree.
● Quantitative polarographic analysis
- determination of Zn by the calibration curve method

4.Chromatography techniques -
● Capillary gas chromatography
- identification of substances in an unknown sample using the Kovets index method a practice
work with internal standard
● Reverse phase high performance liquid chromatography
- determination of phenolic compounds in water samples after pre-separation by solid phase
- determination of caffeine in food samples
● Electrophoresis, Thin Film Chromatography
- optimization of separation conditions and identification of synthetic dyestuffs in food

Recommended or required reading:
PURDEŠOVÁ, A. -- TARAPČÍK, P. -- SÁDECKÁ, J. -- HROBOŇOVÁ, K. -- ŠPÁNIK, I. -- RIEVAJ, M. -- ŠVORC, Ľ. -- MANOVÁ, A. -- ČACHO, F. Praktikum z analytickej chémie. Bratislava : Nakladateľstvo STU, 2011. 242 p. ISBN 978-80-227-3555-1.
BUSTIN, D. -- HLADKY, Z. -- GARAJ, J. Analytická chémia. Alfa, Bratislava: SNTL, Praha, 1987. 744 p.

Language of instruction: slovak or english
Courses evaluation:
Assessed students in total: 379

30,9 %34,8 %26,9 %6,9 %0,5 %0 %
Name of lecturer(s): doc. Ing. Eva Benická, PhD. (instructor)
RNDr. Jana Blaškovičová, PhD. (instructor)
doc. Ing. Katarína Hroboňová, PhD. (person responsible for course)
Ing. Pavel Májek, PhD. (instructor) - slovak, english
Ing. Alena Manová, PhD. (instructor) - slovak, english
doc. Ing. Andrea Purdešová, PhD. (instructor)
Ing. Jozef Sochr, PhD. (instructor)
Ing. Veronika Svitková, PhD. (instructor)
Last modification: 16. 7. 2019
Supervisor: doc. Ing. Katarína Hroboňová, PhD. and programme supervisor

Last modification made by Ing. Tomáš Molnár on 07/16/2019.

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