Information sheet ECTS Syllabus
Course syllabus 426L8_4I - Laboratory Practice in Cell Cultures (FCFT - WS 2019/2020)
|University:||Slovak University of Technology in Bratislava|
|Faculty of Chemical and Food Technology|
Course unit code:
Course unit title:
|Laboratory Practice in Cell Cultures|
Mode of delivery, planned learning activities and teaching methods:
Biochemistry and Biomedical Technologies - master (compulsory), 3. semester
|Level of study:||2.|
Prerequisites for registration:
Aquirement least 56% in from the intended prelims.
|Learning outcomes of the course unit:|
General procedures and practical techniques for cell cultures.
General procedures for cell cultures – design and layout of the laboratory . Work and laboratory safety. General practical procedures for cell cultures. Requirements of cell cultures – the substrate, gas phase , media and supplements, pH, incubation temperature. Experimental subculturing of continuous cell lines grown in monolayer and in suspension. The experimental observation of the effect of serum, pH and incubation temperature on the cell proliferation. Detection of microbial contamination. The practical procedures for preserving and storing living cells. Experimental measurement of viability and cytotoxicity , cell morphology, cell proliferation, cell viability (MTT assay, trypan blue exclusion).
Recommended or required reading:
Language of instruction:
slovak or english
Assessed students in total: 277
Name of lecturer(s):
|doc. Ing. Soňa Jantová, CSc. (examiner, instructor, person responsible for course) - slovak, english|
29. 1. 2019
|Supervisor:||doc. Ing. Soňa Jantová, CSc. and programme supervisor|
Last modification made by Ing. Tomáš Molnár on 01/29/2019.