Oct 23, 2020   11:31 a.m. Alojza
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Course syllabus 426L8_4I - Laboratory Practice in Cell Cultures (FCFT - WS 2019/2020)

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University: Slovak University of Technology in Bratislava
Faculty of Chemical and Food Technology
Course unit code:
Course unit title:
Laboratory Practice in Cell Cultures
Mode of delivery, planned learning activities and teaching methods:
laboratory/construction practice
2 hours weekly (on-site method)

Credits allocated:
Recommended semester/trimester:
Biochemistry and Biomedical Technologies - master (compulsory), 3. semester
Level of study: 2.
Prerequisites for registration:
Assesment methods:
Aquirement least 56% in from the intended prelims.
Learning outcomes of the course unit:
General procedures and practical techniques for cell cultures.
Course contents:
General procedures for cell cultures – design and layout of the laboratory . Work and laboratory safety. General practical procedures for cell cultures. Requirements of cell cultures – the substrate, gas phase , media and supplements, pH, incubation temperature. Experimental subculturing of continuous cell lines grown in monolayer and in suspension. The experimental observation of the effect of serum, pH and incubation temperature on the cell proliferation. Detection of microbial contamination. The practical procedures for preserving and storing living cells. Experimental measurement of viability and cytotoxicity , cell morphology, cell proliferation, cell viability (MTT assay, trypan blue exclusion).

Recommended or required reading:
JANTOVÁ, S. Viabilita, proliferácia a smrť buniek kultivovaných v in vitro podmienakch. Bratislava: Nakladateľstvo STU, 2011. 60 p. ISBN 978-80-227-3464-6.
JANTOVÁ, S. Bunkové kultúry a tkanivové inžinierstvo. Bratislava: Nakladateľstvo STU, 2009. 127 p. ISBN 978-80-227-3204-8.

Language of instruction:
slovak or english
Courses evaluation:
Assessed students in total: 277

70,8 %
12,3 %
9,0 %
5,4 %2,2 %
0,3 %
Name of lecturer(s):
doc. Ing. Soňa Jantová, CSc. (examiner, instructor, person responsible for course) - slovak, english
Last modification:
29. 1. 2019
Supervisor: doc. Ing. Soňa Jantová, CSc. and programme supervisor

Last modification made by Ing. Tomáš Molnár on 01/29/2019.

Type of output: