Oct 26, 2020   2:51 a.m. Demeter
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Course syllabus N426L1_4I - Laboratory of Biochemistry (FCFT - WS 2019/2020)

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University: Slovak University of Technology in Bratislava
Course unit code: N426L1_4I
Course unit title:
Laboratory of Biochemistry
Mode of delivery, planned learning activities and teaching methods:
laboratory/construction practice2 hours weekly (on-site method)

Credits allocated: 2
Recommended semester/trimester:
Biochemistry and Biomedical Technologies - master (compulsory), 1. semester
Biochemistry and Biomedical Technologies - master (compulsory), 1. semester
Level of study:
Prerequisites for registration: none
Assesment methods:
Final assessment will be made by evaluation of protocols and written tests.
Learning outcomes of the course unit:
Student obtains knowledge about methods serving for isolation and analyze of biological macromolecules, isolation cellular structures and measurement of enzyme activities.

Course contents:
- isolation of milk proteins and their analyses by polyacrylamide gel electrophoresis.
isolation of plasmid DNA from Escherichia coli using alkaline lysis.
- estimation of kinetic parameters of purified lactate dehydrogenase.
- isolation of intact chloroplasts from plants and demonstration of the Hill’s reaction.
- isolation of membrane fractions from homogenate of filamentous fungi and determination of activity of respiratory enzymes.
- methods of determination of marker enzyme activities.
Recommended or required reading:
LAKATOŠ, B. -- ŠIMKOVIČ, M. Biochémia: návody na laboratórne cvičenia. Bratislava : Nakladateľstvo STU, 2012. 150 p. ISBN 978-80-227-3793-7.

Language of instruction:
slovak or english
Courses evaluation:
Assessed students in total: 107

26,2 %
24,3 %
28,0 %
14,0 %
4,7 %
2,8 %
Name of lecturer(s):
Ing. Helena Galádová (examiner, instructor)
Ing. Zoltán Polozsányi (examiner, instructor)
doc. Ing. Martin Šimkovič, PhD. (person responsible for course) - slovak, english
Last modification: 29. 1. 2019
doc. Ing. Martin Šimkovič, PhD. and programme supervisor

Last modification made by Ing. Tomáš Molnár on 01/29/2019.

Type of output: