Oct 19, 2020   6:23 p.m. Kristián
Academic information system

Course syllabus N426Z2_4B - Fundamentals of Molecular Biology (FCFT - WS 2019/2020)

     Information sheet          ECTS          Syllabus          

     Slovak          English          

Slovak University of Technology in Bratislava
Faculty: Faculty of Chemical and Food Technology
Course unit code:
Course unit title:
Fundamentals of Molecular Biology
Mode of delivery, planned learning activities and teaching methods:
3 hours weekly (on-site method)

Credits allocated:
Recommended semester/trimester:
Biotechnology - bachelor (compulsory), 5. semester
Food, Nutrition, Cosmetics - bachelor (compulsory), 5. semester
Level of study:
Prerequisites for registration:
Assesment methods:
-- item not defined --
Learning outcomes of the course unit:
Molecular biology and genetics describes the major molecular processes that lie behind most biological events including replication of DNA, transcription and processing of RNA, translation of genetic information into proteins. This course covers areas such as genome structure, DNA replication and control of gene expression in eukaryotes and prokaryotes.
Course contents:
The structure of DNA. Chromosome organization. Regulation of chromatin structure. Noncoding DNA. The structure and versatility of RNA.
DNA replication. DNA polymerases. The replication apparatus. Topoisomerases. The structure of telomeric DNA and telomerase. Telomeres, aging and cancer.
DNA recombination. Transposons. Mutation and repair of DNA.
Transcription in prokaryotes. Regulation of transcription: role of σ (sigma) factors, activators and represors. Two-component system in bacteria. Attenuation. Antitermination.
Translation. Genetic code. Recoding. Function of tmRNA and stringent factors/RelA.
Transcription in eukaryotes. The complexity of the transcriptional apparatus in higher organisms. A focus on Pol II and basal transcription factors. The activation and structure of transcription factors. Transcription factors and eukaryotic gene control.
Heterochromatin and epigenetic control of gene expression.
RNA processing and post-transcriptional gene regulation. Splicing, including alternative splicing; capping; polyadenylation. RNA editing.
Translation. Formation of initiation complex in eukaryotes: control of initiation and phosphorylation of eIF2. RNA interference.
Translocation of proteins - specific signal sequences. Cotranslation translocation into endoplasmic reticulum of proteins. Vesicular transport and sorting of proteins. Traffic between nucleus and cytoplasm. Protein import pathways into mitochondria: TIM and TOM complexes.Degradation of proteins: ubiquitination and proteasome.

Recommended or required reading:
BRAY , D. -- JOHNSON, A. -- ALBERTS, B. Základy buněčné biologie . Ústí nad Labem: Espero, 2005. 740 p. ISBN 80-902906-0-4.

Language of instruction: slovak or english
Courses evaluation:
Assessed students in total: 197

23,4 %
21,8 %
24,9 %
13,2 %
14,7 %2,0 %
Name of lecturer(s):
Ing. Svetlana Kryštofová, PhD. (examiner, instructor, lecturer, tutor) - slovak, english
doc. RNDr. Helena Paulíková, CSc. (person responsible for course) - slovak, english
Last modification:
29. 1. 2019
doc. RNDr. Helena Paulíková, CSc. and programme supervisor

Last modification made by Ing. Tomáš Molnár on 01/29/2019.

Type of output: