23. 10. 2019  17:20 Alojza
Akademický informační systém

Sylabus předmětu 43122_4B - Principles in Recombinant Technologies (FCFT - SS 2019/2020)


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University: Slovak University of Technology in Bratislava
Faculty: Faculty of Chemical and Food Technology
Course unit code: 43122_4B
Course unit title: Principles in Recombinant Technologies
Mode of delivery, planned learning activities and teaching methods:
lecture2 hours weekly (on-site method)

 
Credits allocated: 3
 
Recommended semester/trimester: Biotechnology - bachelor (compulsory), 6. semester
Level of study: 1.
Prerequisites for registration: (passed Fundamentals of General Microbiology (N426Z0_4B) and passed Fundamentals of Biochemistry (N426Z1_4B)) or (passed Microbiology I (42612_4B) and passed Biochemistry I (426B2_4B))
 
Assesment methods:
Presence and successfully done test exams during semester
 
Learning outcomes of the course unit:
To gain the basic knowledge from recombinant technologies and methods used for clonning.
 
Course contents:
Characterization of gene manipulation and its relationship with other fields. DNA and RNA isolation from cells. Bacic enzyme type used in recombinant Technologies, construction of restriction maps. Properties and construction of vector systems, DNA introduction to cells and indentification of recombinant clones. Preparation and application of genomic and cDNA libraries. Techniques of selection and localization of genes in DNA molecules. Principles and anpplication of bacit techniques (gele, electrophoresis, radio- and nonradio-labelling DNA, hybridization, PCR, sequencing DNA). Genecically modified organisms.
 
Recommended or required reading:
Basic:
BROWN, T A. Gene Cloning and DNA analysis : An introduction. 6th edition. Hoboken, NJ 07030-5774, USA: Wiley & Blackwell, 2010. 318 p. ISBN 978-1-4051-8173-0.

 
Language of instruction: slovak or english
 
Notes:
 
Courses evaluation:
Assessed students in total: 589

ABCDEFX
21,1 %17,0 %20,5 %17,3 %19,9 %4,2 %
Name of lecturer(s): doc. Ing. Milan Čertík, PhD. (person responsible for course) - slovak, english
 
Last modification: 21. 2. 2019
Supervisor: doc. Ing. Milan Čertík, PhD. and programme supervisor


Last modification made by Ing. Tomáš Molnár on 02/21/2019.

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