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Course syllabus N426U1_4B - Úvod do molekulovej biológie (FCFT - WS 2019/2020)


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University: Slovak University of Technology in Bratislava
Faculty:
Course unit code: N426U1_4B
Course unit title:
Úvod do molekulovej biológie
Mode of delivery, planned learning activities and teaching methods:
lecture
2 hours weekly (on-site method)

 
Credits allocated:
2
 
Recommended semester/trimester:
-- item not defined --
Level of study:
1.
Prerequisites for registration:
none
 
Assesment methods:
-- item not defined --
 
Learning outcomes of the course unit:
This course covers areas such as genome structure and stability, mechanism of DNA replication and transcription, processing of RNA molecules, translation of genetic information, including control of gene expression in eukaryotes and prokaryotes.
 
Course contents:
The structure of DNA. Chromosome organization. Regulation of chromatin structure. Noncoding DNA. The structure and versatility of RNA.
DNA replication. DNA polymerases. The replication apparatus. Topoisomerases. The structure of telomeric DNA and telomerase. Telomeres, aging and cancer.
DNA recombination. Transposons. Mutation and repair of DNA.
Transcription in prokaryotes. Regulation of transcription: role of σ (sigma) factors, activators and represors. Two-component system in bacteria. Attenuation. Antitermination.
Genetic code and mechanism of translation. Recoding of mRNA. Function of tmRNA and stringent factors/RelA. CRISPR/cas systems in bacteria, and application of CRISPR/cas system for genome editing
Transcription in eukaryotes. The complexity of the transcriptional apparatus in higher organisms. A focus on Pol II and basal transcription factors. The activation and structure of transcription factors. Transcription factors and eukaryotic gene control.
Heterochromatin and epigenetic control of gene expression.
RNA processing: splicing, including alternative splicing; capping; polyadenylation. RNA editing.
Translation. Ribosomes and formation of initiation complex in eukaryotes: control of initiation by phosphorylation of eIF2. RNA interference.
Translocation of proteins - specific signal sequences. Co-translation translocation into endoplasmic reticulum of proteins. Vesicular transport and sorting of proteins. Traffic between nucleus and cytoplasm. Protein import pathways into mitochondria: TIM and TOM complexes. Degradation of proteins: ubiquitination and proteasome.The structure of DNA. Chromosome organization. Regulation of chromatin structure. Noncoding DNA. The structure and versatility of RNA.
DNA replication. DNA polymerases. The replication apparatus. Topoisomerases. The structure of telomeric DNA and telomerase. Telomeres, aging and cancer.
DNA recombination. Transposons. Mutation and repair of DNA.
Transcription in prokaryotes. Regulation of transcription: role of σ (sigma) factors, activators and represors. Two-component system in bacteria. Attenuation. Antitermination.
Genetic code and mechanism of translation. Recoding of mRNA. Function of tmRNA and stringent factors/RelA. CRISPR/cas systems in bacteria, and application of CRISPR/cas system for genome editing
Transcription in eukaryotes. The complexity of the transcriptional apparatus in higher organisms. A focus on Pol II and basal transcription factors. The activation and structure of transcription factors. Transcription factors and eukaryotic gene control.
Heterochromatin and epigenetic control of gene expression.
RNA processing: splicing, including alternative splicing; capping; polyadenylation. RNA editing.
Translation. Ribosomes and formation of initiation complex in eukaryotes: control of initiation by phosphorylation of eIF2. RNA interference.
Translocation of proteins - specific signal sequences. Co-translation translocation into endoplasmic reticulum of proteins. Vesicular transport and sorting of proteins. Traffic between nucleus and cytoplasm. Protein import pathways into mitochondria: TIM and TOM complexes. Degradation of proteins: ubiquitination and proteasome.
 
Recommended or required reading:
Recommended:
ALBERTS B., BRAY D., JOHNSON A Základy buněčné biologie . Ústí nad Labem: Espero, , 2005. 740 s. ISBN 80-902906-0-4

 
Language of instruction: -- item not defined --
 
Notes:
 
Courses evaluation:
Assessed students in total: 0

Name of lecturer(s):
Ing. Svetlana Kryštofová, PhD. (examiner, lecturer, tutor) - slovak, english
doc. RNDr. Helena Paulíková, CSc. (person responsible for course) - slovak, english
 
Last modification:
13. 5. 2020
Supervisor:
doc. RNDr. Helena Paulíková, CSc. and programme supervisor


Last modification made by Samuel Hraško on 05/13/2020.

Type of output: