Oct 28, 2020   4:26 a.m. Dobromila
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Course syllabus 443B0_4D - Biochemistry (FCFT - 2019/2020 - post-graduate studies)

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University: Slovak University of Technology in Bratislava
Course unit code:
Course unit title:
Mode of delivery, planned learning activities and teaching methods:
5 hours weekly (on-site method)

Credits allocated:
Recommended semester/trimester:
Biochemistry - doctoral (compulsory), 1. year
Biochemistry - doctoral (compulsory), 1. year
Biotechnology - doctoral (semi-compulsory), 1. year
Level of study:
Prerequisites for registration:
Assesment methods:
The course will be closed by an examen.
Learning outcomes of the course unit:
The goal of the course is to strengthen the knowledge given in courses Introduction to Biochemistry and Biochemistry and acquire knowledge specific to the topic of PhD thesis.
Course contents:
Chemical composition of cells and organisms. Role of ions, trace elements, and water. Buffers. Energetics of living organisms. Equilibrium and non-equilibrium processes. Chemical equilibria in cells. Coupling of exergonic and endergonic reactions. High-energy bonds. DNA structure and properties DNA denaturation. DNA replication. Types of RNA, their structure and function. Transkription. Genetic code. Enzymic activities of RNA. Amino acids, structure, properties and chemical reactions. Peptide bond. Proteins and polypeptides, their properties and structure. Methods for the study of protein structure. Protein synthesis. Monosaccharides, chirality and stereochemistry. Biologically relevant monosaccharides and their derivatives. Glycosidic bond. Fatty acids, triacylglycerols, waxes, complex lipids, sterols. Enzymes. Enzymic reactions, kinetics. Inhibition of enzyme reactions. Cellular metabolism and its regulation. Model of operon. Regulation by changes in enzyme activities. Glycolysis. Gluconeogenesis. Citrate cycle. Anaplerotic reactions. Glyoxylate cycle. Pentose phosphate pathway. Oxidative phosphorylation. Repiratory chain. Coupling of substrate oxidation with ATP formation. Proton-motive force. Transport of substrates and products across mitochondrial membranes. Fatty acid utilisation and biosynthesis and their enzymologic, energetic and transport aspects. Photosynthetic phosphorylation. CO2 fixation in C3 and C4 plants. Photorespiration. Calvin cycle.
Utilisation and detoxification of ammonia in ureogenetic cycle. General reactions of amino acids. Biosynthesis of canonical amino acids. Metabolism of C1 compounds. Degradation of side chains of canonical amino acids. Ketogenic and glucogenic amino acids. Biosynthetic pathway connected with amino acvid degradation. Biosynthesis and degradation of nucleobases and nucleotides and deoxyribonucleotides. Haem biosynthesis and degradation. Pterine biosynthesis. Metabolism of complex lipids, eicosanoids, isoprenods, steroids, carotenoids and polyketides. Primary and secondary metabolism.Biological membranes. Mechanisms and energetics of biological transport. Biological signals. Reception and transduction of biological signals. Receptors, transduction mechanisms, second messengers and effector mechanism. Hormones. Integration mechanisms in cells and organisms (homeostasis, maintenance of pH, Ca2+, glucose). Blood coagulation. Nerve system and basics of its functions. Light and living organis. Light as source of energy. Molecular mechanism of vision. Responses of organisms to the light pulse. Bioluminiscence. Biochemism of motility and contractility. Cytoskeleton. Muscle and contractile proteins. Metabolism of xenobiotics. The role of oxygen in metabolism. Reactive oxygens species. Detoxication mechanisms and enzymes in cells and organisms.
Recommended or required reading:
DEVLIN, T M. Textbook of Biochemistry with Clinical Correlations. New York : Wiley-Liss, Inc, 1997. 1186 p. ISBN 0-471-15451-2.
ZUBAY, G L. -- PARSON, W W. -- VANCE, D E. Principles of Biochemistry: Volume Two - Metabolism. Dubuque : Wm.C.Brown Publishers, 1995. 624 p. ISBN 0-697-24170-X.
ZUBAY, G L. -- PARSON, W W. -- VANCE, D E. Principles of Biochemistry. Volume One: Energy, Proteins, and Catalysis. Dubuque : Wm.C.Browm Communications, 1995. 224 p. ISBN 0-697-24169-6.
ZUBAY, G L. -- PARSON, W W. -- VANCE, D E. Principles of Biochemistry. Volume Three: Molecular Genetics. Dubuque : Wm.C.Browm Communications, 1995. 625 p. ISBN 0-697-24171-8.

Language of instruction:
slovak and english
Courses evaluation:
Assessed students in total: 96

100,0 %
0 %
Name of lecturer(s):
prof. Ing. Albert Breier, DrSc. (person responsible for course) - slovak, english
Last modification:
26. 4. 2019
prof. Ing. Albert Breier, DrSc. and programme supervisor

Last modification made by Ing. Tomáš Molnár on 04/26/2019.

Type of output: