Information sheet ECTS Syllabus
Course syllabus N400M1_4D - Molecular Biology (FCFT - 2019/2020 - post-graduate studies)
|University:||Slovak University of Technology in Bratislava|
|Course unit code:||N400M1_4D|
Course unit title:
Mode of delivery, planned learning activities and teaching methods:
Biochemistry - doctoral (compulsory), 1. year
Biotechnology - doctoral (semi-compulsory), 1. year
|Level of study:||3.|
|Prerequisites for registration:||none|
The conditions for passing the course are in accordance with the Study Rules of the STU in Bratislava.
|Learning outcomes of the course unit:|
|The lectures are aimed at students interested in furthering their understanding of how cells regulate and coordinate their core activities. The first part will be mainly background of gene regulation in eukaryotes and signalling cellular systems. The second part will focused on signalling defects and human disorders.|
|The structure of DNA. Chromosome organization. Regulation of chromatin structure. Noncoding DNA. The structure and versatility of RNA.
DNA replication. DNA polymerases. The replication apparatus. Topoisomerases. The structure of telomeric DNA and telomerase. Telomeres, aging and cancer.
DNA recombination. Transposons. Mutation and repair of DNA.
Transcription in prokaryotes. Regulation of transcription: role of σ (sigma) factors, activators and represors. Two-component system in bacteria. Attenuation. Antitermination.
Translation. Genetic code. Recoding. Function of tmRNA and stringent factors/RelA.
Transcription in eukaryotes. The complexity of the transcriptional apparatus in higher organisms. A focus on Pol II and basal transcription factors. The activation and structure of transcription factors. Transcription factors and eukaryotic gene control. Heterochromatin and epigenetic control of gene expression.
RNA processing and post-transcriptional gene regulation. Splicing, including alternative splicing; capping; polyadenylation. RNA editing.
Translation. Formation of initiation complex in eukaryotes: control of initiation and phosphorylation of eIF2. RNA interference.
Translocation of proteins - specific signal sequences. Cotranslation translocation into endoplasmic reticulum of proteins. Vesicular transport and sorting of proteins. Traffic between nucleus and cytoplasm. Protein import pathways into mitochondria: TIM and TOM complexes.Degradation of proteins: ubiquitination and proteasome.
Receptors - plasma membrane receptors and intracellular receptors. Proteins of signalling pathways and second messengers. Organization of signalling complexes. Integration of signals. Extracellular matrix and immune system. Signalling in the endocrine system. Principles of cell-cycle regulation. Apoptosis - the necessity for cell death in multicellular organisms. Importance of cell death in human disease - cancer and neurodegenerative diseases.
|Recommended or required reading:|
Language of instruction:
slovak and english
|Assessed students in total: 48|
|Name of lecturer(s):|
|26. 4. 2019|
|Supervisor:||doc. RNDr. Helena Paulíková, CSc. and programme supervisor|
Last modification made by Ing. Tomáš Molnár on 04/26/2019.