Information sheet ECTS Syllabus
Course syllabus N426L1_4I - Laboratory of Biochemistry (FCFT - WS 2020/2021)
|University:||Slovak University of Technology in Bratislava|
|Faculty:||Faculty of Chemical and Food Technology|
|Course unit code:||N426L1_4I|
|Course unit title:||Laboratory of Biochemistry|
|Mode of delivery, planned learning activities and teaching methods:|
|Recommended semester/trimester:||Biochemistry and Biomedical Technologies - master (compulsory), 1. semester|
|Level of study:||2.|
|Prerequisites for registration:||none|
|Final assessment will be made by evaluation of protocols and written tests.|
|Learning outcomes of the course unit:|
|Student obtains knowledge about methods serving for isolation and analyze of biological macromolecules, isolation cellular structures and measurement of enzyme activities.
|- isolation of milk proteins and their analyses by polyacrylamide gel electrophoresis.
isolation of plasmid DNA from Escherichia coli using alkaline lysis.
- estimation of kinetic parameters of purified lactate dehydrogenase.
- isolation of intact chloroplasts from plants and demonstration of the Hill’s reaction.
- isolation of membrane fractions from homogenate of filamentous fungi and determination of activity of respiratory enzymes.
- methods of determination of marker enzyme activities.
|Recommended or required reading:|
|Language of instruction:||slovak or english|
|Assessed students in total: 85|
|Name of lecturer(s):||doc. Ing. Martin Šimkovič, PhD. (person responsible for course) - slovak, english|
|Last modification:||20. 5. 2019|
|Supervisor:||doc. Ing. Martin Šimkovič, PhD. and programme supervisor|
Last modification made by Ing. Tomáš Molnár on 05/20/2019.