Nov 12, 2019   6:58 p.m. Svätopluk
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Course syllabus N426L1_4I - Laboratory of Biochemistry (FCFT - WS 2020/2021)


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University: Slovak University of Technology in Bratislava
Faculty: Faculty of Chemical and Food Technology
Course unit code: N426L1_4I
Course unit title: Laboratory of Biochemistry
Mode of delivery, planned learning activities and teaching methods:
laboratory/construction practice2 hours weekly (on-site method)

 
Credits allocated: 2
 
Recommended semester/trimester: Biochemistry and Biomedical Technologies - master (compulsory), 1. semester
Level of study: 2.
Prerequisites for registration: none
 
Assesment methods:
Final assessment will be made by evaluation of protocols and written tests.
 
Learning outcomes of the course unit:
Student obtains knowledge about methods serving for isolation and analyze of biological macromolecules, isolation cellular structures and measurement of enzyme activities.

 
Course contents:
- isolation of milk proteins and their analyses by polyacrylamide gel electrophoresis.
isolation of plasmid DNA from Escherichia coli using alkaline lysis.
- estimation of kinetic parameters of purified lactate dehydrogenase.
- isolation of intact chloroplasts from plants and demonstration of the Hill’s reaction.
- isolation of membrane fractions from homogenate of filamentous fungi and determination of activity of respiratory enzymes.
- methods of determination of marker enzyme activities.
 
Recommended or required reading:
Basic:
LAKATOŠ, B. -- ŠIMKOVIČ, M. Biochémia: návody na laboratórne cvičenia. Bratislava : Nakladateľstvo STU, 2012. 150 p. ISBN 978-80-227-3793-7.

 
Language of instruction: slovak or english
 
Notes:
 
Courses evaluation:
Assessed students in total: 85

ABCDEFX
31,8 %23,5 %25,9 %12,9 %3,5 %2,4 %
Name of lecturer(s): doc. Ing. Martin Šimkovič, PhD. (person responsible for course) - slovak, english
 
Last modification: 20. 5. 2019
Supervisor: doc. Ing. Martin Šimkovič, PhD. and programme supervisor


Last modification made by Ing. Tomáš Molnár on 05/20/2019.

Type of output: