Oct 24, 2020   11:49 a.m. Kvetoslava
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Course syllabus N431L3_4I - Laboratory of Environmental Biotechnology (FCFT - WS 2020/2021)

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Slovak University of Technology in Bratislava
Course unit code: N431L3_4I
Course unit title: Laboratory of Environmental Biotechnology
Mode of delivery, planned learning activities and teaching methods:
laboratory/construction practice
2 hours weekly (on-site method)

Credits allocated: 2
Recommended semester/trimester: Biotechnology - master (compulsory), 3. semester
Biotechnology - master (compulsory), 3. semester
Level of study:
Prerequisites for registration:
Assesment methods:
Final test
Submission of protocol from each laboratory practice
Classified credit
Learning outcomes of the course unit:
Laboratory methods include basic methods of isolation and identification of microorganisms with the potential degradation ability for selected pollutants of the environment, mainly pentachlorophenol, biphenyl, and polychlorinated biphenyls belonging to the persistent organic pollutants (POPs). Students are working with the real samples of contaminated bottom river sediments. Biodegradation experiments are performed in the defined mineral media artificially contaminated with selected pollutants followed by extraction of studied compounds using Soxhlet apparatus. To identify analyzed compounds gas chromatography, liquid chromatography, and spectrophotometer are used.
Course contents:
1. Introduction.
2. Isolation of cultivable bacterial strains with potential degradation ability under selection pressure.
3. Identification of obtained isolates using Gram test and polymerase chain reaction.
4. Preparation of calibration curves for studied compounds - biphenyl, pentachlorophenol and PCBs. Preparation of calibration curves for biomass evaluation of used bacterial strains.
5. Biodegradation experiments using individual strains and consortium of selected strains.
6. Sonification of cultivation media and extraction of studied compounds with n-hexane.
7. Determination of biodegradation of studied compounds using GC and HPLC and determination of biomass using colony forming units on Petri dishes.

Recommended or required reading:
Language of instruction:
slovak or english
Courses evaluation:
Assessed students in total: 118

60,2 %
23,7 %12,7 %2,5 %
0,9 %
0 %
Name of lecturer(s):
doc. Ing. Katarína Dercová, PhD. (person responsible for course) - slovak, english
Ing. Tatiana Klempová, PhD. (examiner, instructor)
Ing. Veronika Urbaníková (instructor)
Last modification:
20. 4. 2020
doc. Ing. Katarína Dercová, PhD. and programme supervisor

Last modification made by Samuel Hraško on 04/20/2020.

Type of output: