Persons at STU
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|Language of final thesis:||English|
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|Title of the thesis:||Cell wall and its participation in Ca2+ homeostasis of Trichoderma atroviride|
|Summary:||Calcium plays an important role in overall cell functioning and regulating. Usually low cytoplasmic concentration of free Ca2+ (up to 100 nM) can be changed upon stimuli and it is connected to the transfer of Ca2+ ions through the membranes. This project was focusing on participation of cell wall (CW) in Ca2+ homeostasis and the impact of light on the Ca2+-binding capacity of CW. We isolated the Ca2+-binding proteins (CaBP) from CW of Trichoderma atroviride at different stages of development and growth using affinity chromatography. We tried to confirm their presence by autoradiography and by electrophoretic mobility shift assay. Portion of CaBP among CW proteins was only minor, but their future identification could clarify the role of CW in calcium homeostasis and cell signalling in filamentous fungi. The effect of illumination on the Ca2+-binding capacity of CW isolated from mycelium of T. atroviride was estimated by measurement of 45Ca2+ accumulation in CW. The results suggest changes in CW and, simultaneously, calcium redistribution in hyphal surface-structures during the mycelia growth and conidiogenesis.|
|Key words:||light induced conidiation, cell wall, Ca2+ homeostasis, Trichoderma atroviride, calcium binding proteins|
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