Persons at STU
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Basic information about a final thesisAdditional informationAdditional information about the final thesis follows. Click on the language link to display the information in the desired language.
|Language of final thesis:||Slovak|
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|Title of the thesis:||The analysis of covalently bonded oligosaccharides- N- glycans on transferrin|
|Summary:||Glycosylation is one of the most important post translational modifications of protein. Correct arrangement of the monosaccharide units in glycan chains of glycoproteins is essential for a normal course of biochemical processes. This bachelor thesis deals with the preparation of samples for determining congenital disorders of glycosylation - CDG. The main aim of the bachelor thesis is the preparation of glycopeptides glycoprotein, specific transferrin, which subsequent structural analysis by mass spectrometry determines the structure of glycans. Moreover, the bachelor thesis includes a comparison of chromatographic columns HILIC and C18 (ZipTip) as a one step in the sample preparation of glycopeptides prior to the separation by high performance liquid nano HPLC chromatography. This method allows us to prepare the transferrin for subsequent structural analysis by MALDI-TOF mass spectrometry (MS).|
|Key words:||glycosylation, Congenital Disorders of Glycosylation (CDG), transferrin (TF), high performance liquid chromatography (HPLC)|
To display the final thesis assignment form click on the Display the final thesis assignment form icon. The following icons - Final thesis, Thesis appendices, Supervisor's review, Opponent's review - relate to the final thesis and can be downloaded. They could be displayed on condition they have been inserted and are available publicly.Parts of thesis with postponed release:
|–||Final thesis (final thesis appendices) unlimited|
|–||Reviews for final thesis unlimited|