Jan 17, 2020   3:29 p.m. Nataša
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doc. Ing. Eva Benická, PhD.
Identification number: 3371
University e-mail: eva.benicka [at] stuba.sk
Docentka CSc.,PhD. - Institute of Analytical Chemistry (FCFT)

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Basic information

Basic information about a final thesis

Type of thesis: Bachelor thesis
Thesis title:The analysis of covalently bonded oligosaccharides- N- glycans on transferrin
Written by (author): Ing. Nikola Juríčková
Department: Department of Analytical Chemistry (IAC FCFT)
Thesis supervisor: doc. Ing. Eva Benická, PhD.
Opponent:doc. Ing. Jana Sádecká, PhD.
Final thesis progress:Final thesis was successfully defended.

Additional information

Additional information about the final thesis follows. Click on the language link to display the information in the desired language.

Language of final thesis:Slovak

Slovak        English

Title of the thesis:The analysis of covalently bonded oligosaccharides- N- glycans on transferrin
Summary:Glycosylation is one of the most important post translational modifications of protein. Correct arrangement of the monosaccharide units in glycan chains of glycoproteins is essential for a normal course of biochemical processes. This bachelor thesis deals with the preparation of samples for determining congenital disorders of glycosylation - CDG. The main aim of the bachelor thesis is the preparation of glycopeptides glycoprotein, specific transferrin, which subsequent structural analysis by mass spectrometry determines the structure of glycans. Moreover, the bachelor thesis includes a comparison of chromatographic columns HILIC and C18 (ZipTip) as a one step in the sample preparation of glycopeptides prior to the separation by high performance liquid nano HPLC chromatography. This method allows us to prepare the transferrin for subsequent structural analysis by MALDI-TOF mass spectrometry (MS).
Key words:glycosylation, Congenital Disorders of Glycosylation (CDG), transferrin (TF), high performance liquid chromatography (HPLC)

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Parts of thesis with postponed release:

Final thesis (final thesis appendices) unlimited
Reviews for final thesis unlimited