Jan 22, 2020   8:22 a.m. Zora
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doc. Ing. Eva Benická, PhD.
Identification number: 3371
University e-mail: eva.benicka [at] stuba.sk
Docentka CSc.,PhD. - Institute of Analytical Chemistry (FCFT)

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Basic information

Basic information about a final thesis

Type of thesis: Diploma thesis
Thesis title:Structural analysis of avocado fruit polysaccharides (Persea americana, Mill.)
Written by (author): Ing. Nikola Juríčková
Department: Institute of Analytical Chemistry (FCFT)
Thesis supervisor: doc. Ing. Eva Benická, PhD.
Opponent:doc. Ing. Katarína Hroboňová, PhD.
Final thesis progress:Final thesis was successfully defended.

Additional information

Additional information about the final thesis follows. Click on the language link to display the information in the desired language.

Language of final thesis:Slovak

Slovak        English

Title of the thesis:Structural analysis of avocado fruit polysaccharides (Persea americana, Mill.)
Summary:The analysis of polysaccharide structures is of general interest but also of industrial relevance as the structure and properties of polysaccharides are closely related. Polysaccharide analysis requires specialized techniques, which differ from those methods used for the characterization of small molecules. Methylation analysis is the most widely used and highly effective method for structure determination of polysaccharides. Despite its advantages we also comes into the contact with shortcomings such as: loss of information and the problem with analysis of ketosis. Because of this, it is necessary to use another method for determining the structure of polysaccharides which is called reductive cleavage. The structure of polysaccharides in avocado was determinated in this thesis. Both mentioned methods were used for this analysis. Higher comfort with determining the structure of polysaccharides was achieved by comparing their results. However, integral part of this analysis was the determination of molecular weight polysaccharides and their distribution by GPC (gel permeation chromatography).
Key words:avocado, structural analysis of polysaccharides, methylation analysis, reductive cleavage, GPC

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