Jan 26, 2020   4:34 a.m. Tamara
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Ing. Kristína Markošová, PhD.
Odborná asistentka CSc.,PhD. - Institute of Biotechnology (FCFT) - interrupted

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Basic information

Basic information about a final thesis

Type of thesis: Bachelor thesis
Thesis title:Production of Redox Enzymes and Their Application in Cofactor Regeneration
Written by (author): Ing. Zuzana Rosenbergová
Department: Institute of Biotechnology (FCFT)
Thesis supervisor: Ing. Kristína Markošová, PhD.
Opponent:Ing. Tomáš Molnár
Final thesis progress:Final thesis was successfully defended.

Additional information

Additional information about the final thesis follows. Click on the language link to display the information in the desired language.

Language of final thesis:Slovak

Slovak        English

Title of the thesis:Production of Redox Enzymes and Their Application in Cofactor Regeneration
Summary:This bachelor thesis is focused on enzymatic regeneration of cofactors nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) using the enzyme glucose dehydrogenase. Glucose dehydrogenase belongs to the class of oxidoreductases and catalyzes the conversion of D-glucose to D-glucono-1,5-lactone using NAD(P)+ as a cofactor, which is reduced during the reaction and can be used as a cofactor for a different enzyme. The experimental part of this thesis was focused on the production of glucose dehydrogenase by recombinant bacteria Escherichia coli, on the purification of the produced enzyme by affinity chromatography (FPLC) and on its characterization. The affinity towards NAD+ and NADP+ was compared and pH profiles, temperature profiles and kinetic parameters of the isolated glucose dehydrogenase were determined.
Key words:glucose dehydrogenase, cofactor regeneration, NAD(P)+, Escherichia coli

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Parts of thesis with postponed release:

Final thesis (final thesis appendices) unlimited
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