Persons at STU
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Basic information about a final thesis
|Type of thesis:||Bachelor thesis|
Latrophilin-1 - a new leukemic cell biomarker and its impact on medical biotechnology
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|Final thesis was successfully defended.|
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Title of the thesis:
|Latrophilin-1 - a new leukemic cell biomarker and its impact on medical biotechnology|
Acute Myeloid Leukemia (AML) is an oncological disease whose treatment is complicated by the development of multidrug resistance (MDR). Because of MDR, leukemic cells become insensitive to multiple administered drugs. One of the major mechanisms of MDR development represents the overexpression of P-glycoprotein (P-gp), which belongs to the ABC transporter family. Recently, we have found that expression of novel potential biomarker of AML – latrophilin-1 (LPHN1) is downregulated in P-gp positive cells in comparison with their sensitive counterparts. LPHN1 is probably the regulator of Tim-3 and galectin-9 (Gal-9) proteins expression and secretion, which are possibly involved in immune escape of leukemic cells. The aim of this bachelor thesis was to monitor the expression of Tim-3 and Gal-9 in sensitive acute myeloid leukemia cell lines (SKM-1 and MOLM-13) and their resistant cell sublines (SKM-1/VCR and MOLM-13/VCR). We have found that the HAVCR2 gene encoding Tim-3 is expressed in sensitive lines, but higher expression of this gene occurs in both resistant sublines, in which P-gp is overexpressed. Protein expression of Tim-3 also increases in both resistant sublines. In the case of the LGALS9 gene expression (encoding Gal-9), we have registered its transcript in sensitive lines and downregulation of this gene transcription is present in both resistant sublines. Protein level of Gal-9 expression also decreases in resistant sublines.
|Key words:||acute myeloid leukemia, galectin-9, latrophilin-1, P-glycoprotein, Tim-3|
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