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Development of new immobilized biooxidation systems based on hyperoxygenated whole cell/enzyme biocatalysts for novel white biotechnologies.

Supervisor: prof. Ing. Michal Rosenberg, PhD.

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Project description:The project will solve actual problems of bioxidations, which are perspective field of "white" biotechnologies. A broader spectrum of enzyme and whole cell catalysts such as oxidases, (mono)oxygenases and oxidoreductases will be used. The criterion of success will be an efficient catalysis of the biooxidation of synthetic, prochiral substrates to enantiomeric products. Catalytic efficiency will be achieved by co-immobilization of biocatalysts with new, synthetic and water-soluble oxygen carriers. The co-immobilization will be performed by controlled bioencapsulation producing polyelectrolyte capsules with regular spherical shape, narrow size distribution, controlled membrane thickness and size of pores. This original technique should rise the stability of recombinant DNA in biocatalysts and reduce the risk of contamination. Novel NTP/ATP coenzyme-regenerating systems utilizing polyphosphate kinases and polyphosphates as co-substrates will be improved. Original hybrid enzymes which combine extreme(thermo)philic properties with biospecific saccharide-binding affinity will be developed. Achieved results will be utilized for construction of original (amperometric) biosensors and for amplification of their signal.
Kind of project:VEGA ()
Department:Department of Biochemical Technology (IBT FCFT)
Project identification:1/4452/07
Project status:Successfully completed
Project start date :01. 01. 2007
Project close date:31. 12. 2009
Number of workers in the project:2
Number of official workers in the project:0